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Evanescent field shapes excitation profile under axial epi-illumination

Axial epi-illuminating light transmitting a [Formula: see text]-numerical-aperture microscope objective creates an excitation volume at focus with size and shape dictated by diffraction and due to refraction by the objective and by the coverslip interface separating a specimen in aqueous buffer from...

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Bibliografiske detaljer
Hovedforfatter: Burghardt, Thomas P.
Format: Artigo
Sprog:Inglês
Udgivet: Society of Photo-Optical Instrumentation Engineers 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3381040/
https://ncbi.nlm.nih.gov/pubmed/22734777
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/1.JBO.17.6.066021
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