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Axial localization with modulated-illumination extended-depth-of-field microscopy

High-speed volumetric imaging represents a challenge in microscopy applications. We demonstrate a technique for acquiring volumetric images based on the extended depth of field microscopy with a fast focal scan and modulated illumination. By combining two frames with different illumination ramps, we...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Shain, William J., Vickers, Nicholas A., Li, Jiang, Han, Xue, Bifano, Thomas, Mertz, Jerome
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5905922/
https://ncbi.nlm.nih.gov/pubmed/29675318
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.9.001771
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