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A simple assay for 3-deoxy-d-manno-octulosonate cytidylyltransferase (KdsB) and its use as a pathway screen
This paper describes the adaptation of a simple colorimetric assay for inorganic pyrophosphate to the enzyme 3-deoxy-d-manno-octulosonate cytidylyltransferase(CMP-KDO synthetase, KdsB, E.C. 2.7.7.38), a key enzyme in the biosynthesis of lipopolysaccharide (LPS) in Gram-negative organisms. This assay...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2011
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3380378/ https://ncbi.nlm.nih.gov/pubmed/21669179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.05.022 |
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