Functional and biochemical characterization of a recombinant Arabidopsis thaliana 3-deoxy-D-manno-octulosonate 8-phosphate synthase

An open reading frame, encoding for KDOPS (3-deoxy-D-manno-octulosonate 8-phosphate synthase), from Arabidopsis thaliana was cloned into a T7-driven expression vector. The protein was overexpressed in Escherichia coli and purified to homogeneity. Recombinant A. thaliana KDOPS, in solution, displays...

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Bibliographic Details
Main Authors: Wu, Jing, Patel, Mayur A., Sundaram, Appavu K., Woodard, Ronald W.
Format: Artigo
Language:Inglês
Published: Portland Press Ltd. 2004
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1133776/
https://ncbi.nlm.nih.gov/pubmed/15070398
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20040207
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