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Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences
Microbial community profiling using 16S rRNA gene sequences requires accurate taxonomy assignments. ‘Universal' primers target conserved sequences and amplify sequences from many taxa, but they provide variable coverage of different environments, and regions of the rRNA gene differ in taxonomic...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3379642/ https://ncbi.nlm.nih.gov/pubmed/22237546 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ismej.2011.208 |
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