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Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences

Microbial community profiling using 16S rRNA gene sequences requires accurate taxonomy assignments. ‘Universal' primers target conserved sequences and amplify sequences from many taxa, but they provide variable coverage of different environments, and regions of the rRNA gene differ in taxonomic...

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Detalhes bibliográficos
Main Authors: Soergel, David A W, Dey, Neelendu, Knight, Rob, Brenner, Steven E
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3379642/
https://ncbi.nlm.nih.gov/pubmed/22237546
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ismej.2011.208
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