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Fluorescence Fluctuation Spectroscopy Enables Quantitative Imaging of Single mRNAs in Living Cells

Imaging mRNA with single-molecule sensitivity in live cells has become an indispensable tool for quantitatively studying RNA biology. The MS2 system has been extensively used due to its unique simplicity and sensitivity. However, the levels of the coat protein needed for consistent labeling of mRNAs...

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Autors principals: Wu, Bin, Chao, Jeffrey A., Singer, Robert H.
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3379624/
https://ncbi.nlm.nih.gov/pubmed/22735544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.05.017
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