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Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry

The modification of RNA with fluorophores, affinity tags and reactive moieties is of enormous utility for studying RNA localization, structure and dynamics as well as diverse biological phenomena involving RNA as an interacting partner. Here we report a labeling approach in which the RNA of interest...

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Détails bibliographiques
Auteurs principaux: Winz, Marie-Luise, Samanta, Ayan, Benzinger, Dirk, Jäschke, Andres
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3378897/
https://ncbi.nlm.nih.gov/pubmed/22344697
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks062
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