Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

Herein we present a protocol of reprogramming human adult fibroblasts into human induced pluripotent stem cells (hiPSC) using retroviral vectors encoding Oct3/4, Sox2, Klf4 and c-myc (OSKM) in the presence of sodium butyrate (1-3). We used this method to reprogram late passage (>p10) human adult...

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Bibliographic Details
Main Authors: Polak, Urszula, Hirsch, Calley, Ku, Sherman, Gottesfeld, Joel, Dent, Sharon Y.R., Napierala, Marek
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2012
Subjects:
Developmental Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3376935/
https://ncbi.nlm.nih.gov/pubmed/22370855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3416
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