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Identification and removal of nitroxide spin label contaminant: Impact on PRE studies of α-helical membrane proteins in detergent
NMR paramagnetic relaxation enhancement (PRE) provides long-range distance constraints (∼15–25 Å) that can be critical to determining overall protein topology, especially where long-range NOE information is unavailable such as in the case of larger proteins that require deuteration. However, several...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Wiley Subscription Services, Inc., A Wiley Company
2012
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3375759/ https://ncbi.nlm.nih.gov/pubmed/22389096 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2038 |
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