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Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgL

The periplasmic alginate lyase AlgL is essential for the synthesis and export of the exopolysaccharide alginate in Pseudomonas sp. and also plays a role in its depolymerization. P. aeruginosa PAO1 AlgL has been overexpressed and purified and diffraction-quality crystals were grown using the hanging-...

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Библиографические подробности
Главные авторы: Wolfram, Francis, Arora, Kritica, Robinson, Howard, Neculai, Ana Mirela, Yip, Patrick, Howell, P. Lynne
Формат: Artigo
Язык:Inglês
Опубликовано: International Union of Crystallography 2012
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3374518/
https://ncbi.nlm.nih.gov/pubmed/22691793
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112012808
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