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Expression, refolding, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgE
AlgE is an outer membrane protein present in alginate-producing (mucoid) Pseudomonas aeruginosa. AlgE has been overexpressed in insoluble inclusion bodies, purified under denaturing conditions and refolded in a buffer containing decyl β-d-maltopyranoside. Purified refolded AlgE was detergent-exchang...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2675585/ https://ncbi.nlm.nih.gov/pubmed/19407377 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910901094X |
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