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Conformational consequences of ionization of Lys, Asp and Glu buried at position 66 in staphylococcal nuclease
The pK(a) values measured previously for the internal Lys-66, Asp-66 and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculation...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3373020/ https://ncbi.nlm.nih.gov/pubmed/20329780 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi902114m |
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