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Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

Summary: The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction methods and undisputedly gave rise to a palette of ingenious techniques that are constantly pushing further the limits of the original method. Sensitivity and selectivity have improved because of various...

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Detalhes bibliográficos
Main Authors: Stynen, Bram, Tournu, Hélène, Tavernier, Jan, Van Dijck, Patrick
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3372256/
https://ncbi.nlm.nih.gov/pubmed/22688816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MMBR.05021-11
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