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In Vitro Reconstitution of the Complete Clostridium thermocellum Cellulosome and Synergistic Activity on Crystalline Cellulose
Artificial cellulase complexes active on crystalline cellulose were reconstituted in vitro from a native mix of cellulosomal enzymes and CipA scaffoldin. Enzymes containing dockerin modules for binding to the corresponding cohesin modules were prepared from culture supernatants of a C. thermocellum...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3370548/ https://ncbi.nlm.nih.gov/pubmed/22522677 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07959-11 |
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