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In Vitro Reconstitution of the Complete Clostridium thermocellum Cellulosome and Synergistic Activity on Crystalline Cellulose

Artificial cellulase complexes active on crystalline cellulose were reconstituted in vitro from a native mix of cellulosomal enzymes and CipA scaffoldin. Enzymes containing dockerin modules for binding to the corresponding cohesin modules were prepared from culture supernatants of a C. thermocellum...

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Detalhes bibliográficos
Main Authors: Krauss, Jan, Zverlov, Vladimir V., Schwarz, Wolfgang H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3370548/
https://ncbi.nlm.nih.gov/pubmed/22522677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07959-11
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