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A Rapid Cloning Method Employing Orthogonal End Protection
We describe a novel in vitro cloning strategy that combines standard tools in molecular biology with a basic protecting group concept to create a versatile framework for the rapid and seamless assembly of modular DNA building blocks into functional open reading frames. Analogous to chemical synthesi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3369885/ https://ncbi.nlm.nih.gov/pubmed/22685543 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037617 |
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