Enzymatic synthesis of DNA strands containing α-L-LNA (α-L-configured locked nucleic acid) thymine nucleotides

We describe the first enzymatic incorporation of an α-L-LNA nucleotide into an oligonucleotide. It was found that the 5′-triphosphate of α-L-LNA is a substrate for the DNA polymerases KOD, 9°N(m), Phusion and HIV RT. Three dispersed α-L-LNA thymine nucleotides can be incorporated into DNA strands by...

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Hlavní autoři: Højland, Torben, Veedu, Rakesh N., Vester, Birte, Wengel, Jesper
Médium: Artigo
Jazyk:Inglês
Vydáno: Landes Bioscience 2012
Témata:
Research Paper
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3368812/
https://ncbi.nlm.nih.gov/pubmed/22679529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/adna.19272
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