High efficiency transformation of E. coli by high voltage electroporation.
E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 an...
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| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1988
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336852/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3041370/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.13.6127 |
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