Wordt geladen...

High efficiency transformation of E. coli by high voltage electroporation.

E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 an...

Volledige beschrijving

Bewaard in:
Bibliografische gegevens
Hoofdauteurs: Dower, W J, Miller, J F, Ragsdale, C W
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1988
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC336852/
https://ncbi.nlm.nih.gov/pubmed/3041370
Tags: Voeg label toe
Geen labels, Wees de eerste die dit record labelt!