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High efficiency transformation of E. coli by high voltage electroporation.
E. coli can be transformed to extremely high efficiencies by subjecting a mixture of cells and DNA to brief but intense electrical fields of exponential decay waveform (electroporation). We have obtained 10(9) to 10(10) transformants/micrograms with strains LE392 and DH5 alpha, and plasmids pUC18 an...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1988
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC336852/ https://ncbi.nlm.nih.gov/pubmed/3041370 |
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