Label-Free Quantitation of Protein Modifications by Pseudo Selected Reaction Monitoring with Internal Reference Peptides

[Image: see text] Liquid chromatography tandem mass spectrometry (LC–MS/MS) based methods provide powerful tools for the quantitative analysis of modified proteins. We have developed a label-free approach using internal reference peptides (IRP) from the target protein for signal normalization withou...

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Detalhes bibliográficos
Main Authors: Sherrod, Stacy D., Myers, Matthew V., Li, Ming, Myers, Jeremy S., Carpenter, Kristin L., MacLean, Brendan, MacCoss, Michael J., Liebler, Daniel C., Ham, Amy-Joan L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2012
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3368409/
https://ncbi.nlm.nih.gov/pubmed/22559222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr201240a
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