Search for the optimal sequence of the ribosome binding site by random oligonucleotide-directed mutagenesis.
Synthetic DNA duplexes corresponding to the ribosome binding site (RBS) were synthesized through the phosphite method on solid support. The synthetic RBS DNA with partial random sequences was inserted into an appropriate site between the lpp-lac promoter and the beta-galactosidase structural gene in...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1988
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336718/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2455279/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.11.5075 |
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