The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
In solutions of tetraalkylammonium salts the melting temperature of oligonucleotide duplexes is independent of nucleotide sequence and thus GC content. Data quantitating the destabilizing effects of various mismatches in these solvents are also presented. The results are in accord with theories on D...
Spremljeno u:
| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1988
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC336654/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3380691/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.10.4637 |
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