Structural and Kinetic Insights Reveal That the Amino Acid Pair Gln-228/Asn-254 Modulates the Transfructosylating Specificity of Schwanniomyces occidentalis β-Fructofuranosidase, an Enzyme That Produces Prebiotics

Schwanniomyces occidentalis β-fructofuranosidase (Ffase) is a GH32 dimeric enzyme that releases fructose from the nonreducing end of various oligosaccharides and essential storage fructans such as inulin. It also catalyzes the transfer of a fructosyl unit to an acceptor producing 6-kestose and 1-kes...

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Detalhes bibliográficos
Main Authors: Álvaro-Benito, Miguel, Sainz-Polo, M. Angela, González-Pérez, David, González, Beatriz, Plou, Francisco J., Fernández-Lobato, María, Sanz-Aparicio, Julia
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2012
Assuntos:
Protein Structure and Folding
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3366002/
https://ncbi.nlm.nih.gov/pubmed/22511773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.355503
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