New Insights into the Fructosyltransferase Activity of Schwanniomyces occidentalis β-Fructofuranosidase, Emerging from Nonconventional Codon Usage and Directed Mutation

Schwanniomyces occidentalis β-fructofuranosidase (Ffase) releases β-fructose from the nonreducing ends of β-fructans and synthesizes 6-kestose and 1-kestose, both considered prebiotic fructooligosaccharides. Analyzing the amino acid sequence of this protein revealed that it includes a serine instead...

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Detalhes bibliográficos
Main Authors: Álvaro-Benito, Miguel, de Abreu, Miguel, Portillo, Francisco, Sanz-Aparicio, Julia, Fernández-Lobato, María
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2976189/
https://ncbi.nlm.nih.gov/pubmed/20851958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01614-10
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