Single-step inline hydroxyapatite enrichment facilitates identification and quantitation of phosphopeptides from mass-limited proteomes with MudPIT

Herein we report the characterization and optimization of single-step inline enrichment of phosphopeptides directly from small amounts of whole cell and tissue lysates (100 – 500 μg) using a hydroxyapatite (HAP) microcolumn and Multidimensional Protein Identification Technology (MudPIT). In comparis...

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Bibliografiske detaljer
Main Authors: Fonslow, Bryan R., Niessen, Sherry M., Singh, Meha, Wong, Catherine C., Xu, Tao, Carvalho, Paulo C., Choi, Jeong, Park, Sung Kyu, Yates, John R.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3365658/
https://ncbi.nlm.nih.gov/pubmed/22509746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr300200x
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