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Partial transfection of cells using laminar flows in microchannels

This manuscript describes a convenient method for partial transfection using a Y-shaped microchannel polydimethylsiloxane (PDMS)-glass chip and on-chip cationic lipid-mediated transfection. Enhanced green fluorescent protein genes (pEGFP-N2) were introduced into the COS-7 cells cultured in half of t...

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Bibliografische gegevens
Hoofdauteurs: Li, Lei, Nie, Yong, Shi, Xuetao, Wu, Hongkai, Ye, Datian, Chen, Hongda
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Institute of Physics 2011
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3364838/
https://ncbi.nlm.nih.gov/pubmed/22662050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.3643827
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