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Fluorescence Polarization and Fluctuation Analysis Monitors Subunit Proximity, Stoichiometry, and Protein Complex Hydrodynamics

Förster resonance energy transfer (FRET) microscopy is frequently used to study protein interactions and conformational changes in living cells. The utility of FRET is limited by false positive and negative signals. To overcome these limitations we have developed Fluorescence Polarization and Fluctu...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Nguyen, Tuan A., Sarkar, Pabak, Veetil, Jithesh V., Koushik, Srinagesh V., Vogel, Steven S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2012
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3364239/
https://ncbi.nlm.nih.gov/pubmed/22666486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0038209
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