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ZyFISH: A Simple, Rapid and Reliable Zygosity Assay for Transgenic Mice

Microinjection of DNA constructs into fertilized mouse oocytes typically results in random transgene integration at a single genomic locus. The resulting transgenic founders can be used to establish hemizygous transgenic mouse lines. However, practical and experimental reasons often require that suc...

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Detalhes bibliográficos
Main Authors: McHugh, Donal, O’Connor, Tracy, Bremer, Juliane, Aguzzi, Adriano
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3362593/
https://ncbi.nlm.nih.gov/pubmed/22666404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037881
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