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ePAT: A simple method to tag adenylated RNA to measure poly(A)-tail length and other 3′ RACE applications

The addition of a poly(A)-tail to the 3′ termini of RNA molecules influences stability, nuclear export, and efficiency of translation. In the cytoplasm, dynamic changes in the length of the poly(A)-tail have long been recognized as reflective of the switch between translational silence and activatio...

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Autors principals: Jänicke, Amrei, Vancuylenberg, John, Boag, Peter R., Traven, Ana, Beilharz, Traude H.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3358650/
https://ncbi.nlm.nih.gov/pubmed/22543866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.031898.111
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