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An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

The real-time PCR diagnostics for avian influenza virus H5N1 in tissue specimens are often suboptimal, since naturally occurring PCR inhibitors present in samples, or unanticipated match of primer to unsequenced species' genome. With the principal aim of optimizing the SYBR Green real-time PCR...

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Kaydedildi:
Detaylı Bibliyografya
Asıl Yazarlar: Zhan, LingJun, Bao, LinLin, Li, FengDi, Lv, Qi, Xu, LiLi, Qin, Chuan
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: The Scientific World Journal 2012
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3356745/
https://ncbi.nlm.nih.gov/pubmed/22645461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/2012/907095
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