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An Optimized Real-Time PCR to Avoid Species-/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues

The real-time PCR diagnostics for avian influenza virus H5N1 in tissue specimens are often suboptimal, since naturally occurring PCR inhibitors present in samples, or unanticipated match of primer to unsequenced species' genome. With the principal aim of optimizing the SYBR Green real-time PCR...

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Autors principals: Zhan, LingJun, Bao, LinLin, Li, FengDi, Lv, Qi, Xu, LiLi, Qin, Chuan
Format: Artigo
Idioma:Inglês
Publicat: The Scientific World Journal 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3356745/
https://ncbi.nlm.nih.gov/pubmed/22645461
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1100/2012/907095
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