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Expression of an Epitope-Tagged Virulence Protein in Rickettsia parkeri Using Transposon Insertion

Despite recent advances in our ability to genetically manipulate Rickettsia, little has been done to employ genetic tools to study the expression and localization of Rickettsia virulence proteins. Using a mariner-based Himar1 transposition system, we expressed an epitope-tagged variant of the actin...

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Detalhes bibliográficos
Main Authors: Welch, Matthew D., Reed, Shawna C. O., Lamason, Rebecca L., Serio, Alisa W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3356282/
https://ncbi.nlm.nih.gov/pubmed/22624012
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0037310
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