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Ribonuclease S Dynamics Measured Using a Nitrile Label with 2D IR Vibrational Echo Spectroscopy

A nitrile labeled amino acid, p-cyanophenylalanine, is introduced near the active site of the semisynthetic enzyme ribonuclease S to serve as a probe of protein dynamics and fluctuations. Ribonuclease S is the limited proteolysis product of subtilisin acting on ribonuclease A, and consists of a smal...

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Detalhes bibliográficos
Main Authors: Bagchi, Sayan, Boxer, Steven G., Fayer, M. D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3354990/
https://ncbi.nlm.nih.gov/pubmed/22417088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp2122856
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