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Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.
Tetanus toxin fragment C had been previously expressed in Escherichia coli at 3-4% cell protein. The codon bias for tetanus toxin in Clostridium tetani is very different from that of highly expressed homologous genes in E. coli, resulting in the presence of many rare E. coli codons in the sequence e...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1989
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC335293/ https://ncbi.nlm.nih.gov/pubmed/2690015 |
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