Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.
Tetanus toxin fragment C had been previously expressed in Escherichia coli at 3-4% cell protein. The codon bias for tetanus toxin in Clostridium tetani is very different from that of highly expressed homologous genes in E. coli, resulting in the presence of many rare E. coli codons in the sequence e...
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| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1989
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335293/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2690015/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.24.10191 |
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