Cloning, characterization and heterologous expression of the SmaI restriction-modification system.

The genes coding for the class-II Serratia marcescens restriction-modification system have been cloned and expressed in E. coli. Recombinant clones, restricted incoming phage only poorly; the recombinant plasmids, however, became fully modified in vivo, i.e. completely resistant against digestion wi...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Heidmann, S, Seifert, W, Kessler, C, Domdey, H
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1989
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335213/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2690008/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.23.9783
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