Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli.
The MspI restriction-modification system, which recognizes the sequence 5'-CCGG-3', has been previously cloned and sequenced (1). We subcloned the methyltransferase gene (M.MspI) downstream of the ptac promoter in the multicopy vector pUC119 and overexpressed it in E. coli. Upon induction with IPTG,...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1992
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312241/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1579450/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.7.1579 |
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