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The spectrum of mutations generated by passage of a hydrogen peroxide damaged shuttle vector plasmid through a mammalian host.

Treatment of a plasmid shuttle vector (pZ189) with a combination of hydrogen peroxide and a ferric iron/EDTA complex prior to transfection and passage in simian (CV-1) cells increases the frequency of mutations at the supF locus by up to 60-fold over the spontaneous background. This increase in muta...

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Autors principals: Moraes, E C, Keyse, S M, Pidoux, M, Tyrrell, R M
Format: Artigo
Idioma:Inglês
Publicat: 1989
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC334965/
https://ncbi.nlm.nih.gov/pubmed/2682525
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