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In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.
The method for detecting the small fraction (1%) of transcriptionally active SV40 minichromosomes, in the presence of the bulk minichromosomes, (14) has been applied to directly analyze the topology of transcribed and non-transcribed minichromosomal DNA. We show here that DNA of both transcribed and...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
1988
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334726/ https://ncbi.nlm.nih.gov/pubmed/2830595 |
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