In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.
The method for detecting the small fraction (1%) of transcriptionally active SV40 minichromosomes, in the presence of the bulk minichromosomes, (14) has been applied to directly analyze the topology of transcribed and non-transcribed minichromosomal DNA. We show here that DNA of both transcribed and...
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| Опубликовано в:: | Nucleic Acids Res |
|---|---|
| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1988
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334726/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2830595/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.3.895 |
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