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Identification of sequence-specific DNA-binding factors by label transfer: application to the adenovirus-2 major late promoter.
A method of affinity labelling proteins specifically associated with DNA target sequences is proposed. The method utilizes covalent UV-crosslinking of proteins to highly labelled DNA (e.g. in crude cell or nuclear extracts) followed by degradation of the DNA to short oligonucleotides. Proteins selec...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1988
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC334676/ https://ncbi.nlm.nih.gov/pubmed/3340547 |
| Tags: |
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