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Identification of sequence-specific DNA-binding factors by label transfer: application to the adenovirus-2 major late promoter.

A method of affinity labelling proteins specifically associated with DNA target sequences is proposed. The method utilizes covalent UV-crosslinking of proteins to highly labelled DNA (e.g. in crude cell or nuclear extracts) followed by degradation of the DNA to short oligonucleotides. Proteins selec...

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Detalhes bibliográficos
Main Authors: Nikolaev, L G, Glotov, B O, Belyavsky, A V, Grachev, S A, Levin, A V
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC334676/
https://ncbi.nlm.nih.gov/pubmed/3340547
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