Cytoplasmic pH Response to Acid Stress in Individual Cells of Escherichia coli and Bacillus subtilis Observed by Fluorescence Ratio Imaging Microscopy

The ability of Escherichia coli and Bacillus subtilis to regulate their cytoplasmic pH is well studied in cell suspensions but is poorly understood in individual adherent cells and biofilms. We observed the cytoplasmic pH of individual cells using ratiometric pHluorin. A standard curve equating the...

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Bibliografiske detaljer
Main Authors: Martinez, Keith A., Kitko, Ryan D., Mershon, J. Patrick, Adcox, Haley E., Malek, Kotiba A., Berkmen, Melanie B., Slonczewski, Joan L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2012
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3346368/
https://ncbi.nlm.nih.gov/pubmed/22427503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00354-12
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