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pH of the Cytoplasm and Periplasm of Escherichia coli: Rapid Measurement by Green Fluorescent Protein Fluorimetry

Cytoplasmic pH and periplasmic pH of Escherichia coli cells in suspension were observed with 4-s time resolution using fluorimetry of TorA-green fluorescent protein mutant 3* (TorA-GFPmut3*) and TetR-yellow fluorescent protein. Fluorescence intensity was correlated with pH using cell suspensions con...

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Detalhes bibliográficos
Main Authors: Wilks, Jessica C., Slonczewski, Joan L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1951819/
https://ncbi.nlm.nih.gov/pubmed/17545292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00615-07
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