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Cooperation between Catalytic and DNA-binding Domains Enhances Thermostability and Supports DNA Synthesis at Higher Temperatures by Thermostable DNA Polymerases

We have previously introduced a general kinetic approach for comparative study of processivity, thermostability, and resistance to inhibitors of DNA polymerases (Pavlov et. al., (2002) Proc. Natl. Acad. Sci. USA 99, 13510–13515). The proposed method was successfully applied to characterize hybrid DN...

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Detalhes bibliográficos
Main Authors: Pavlov, Andrey R., Pavlova, Nadejda V., Kozyavkin, Sergei A., Slesarev, Alexei I.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3345285/
https://ncbi.nlm.nih.gov/pubmed/22320201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2014807
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