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Comparison of the Human and Bovine Milk N-Glycome via High-Performance Microfluidic Chip Liquid Chromatography and Tandem Mass Spectrometry

The isolation of whey proteins from human and bovine milks followed by profiling of their entire N-glycan repertoire is described. Whey proteins resulting from centrifugation and ethanol precipitation of milk were treated with PNGase F to release protein-bound N-glycans. Once released, N-glycans wer...

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Autors principals: Nwosu, Charles C., Aldredge, Danielle L., Lee, Hyeyoung, Lerno, Larry A., Zivkovic, Angela M., German, J. Bruce, Lebrilla, Carlito B.
Format: Artigo
Idioma:Inglês
Publicat: 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3345083/
https://ncbi.nlm.nih.gov/pubmed/22439776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr300008u
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