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Oligonucleotide probes detect splicing variants in situ in Drosophila embryos.

We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and II...

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Bibliographic Details
Main Authors: Artero, R D, Akam, M, Pérez-Alonso, M
Format: Artigo
Language:Inglês
Published: 1992
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC334403/
https://ncbi.nlm.nih.gov/pubmed/1454531
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