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Oligonucleotide probes detect splicing variants in situ in Drosophila embryos.
We describe a method for the in situ detection of specific splicing variants. The method is based on the use of antisense oligonucleotides designed to span splice junctions labelled with digoxigenin by terminal transferase tailing. We find that the spatial patterns of Ubx splicing variants Ia and II...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1992
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334403/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1454531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.21.5687 |
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