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Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples.

We report a modification to the peak-height encoded DNA sequencing technique of Tabor and Richardson. As in the original protocol, the sequencing reaction uses modified T7 polymerase with manganese rather than magnesium to produce very uniform incorporation of each dideoxynucleoside. To improve sequ...

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Bibliografische gegevens
Gepubliceerd in:Nucleic Acids Res
Hoofdauteurs: Chen, D, Harke, H R, Dovichi, N J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 1992
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC334245/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1408803/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.18.4873
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